Detection of genomic regions differentiating two closely related oak species Quercus petraea (Matt.) Liebl. and Quercus robur L

Publication Type:Journal Article
Year of Publication:1997
Authors:Bod, Joandet, S., Laigret, F., Kremer, A.
Date Published:1997

Genomic regions differentiating Quercus petraea and Quercus robur were detected by screening2800 PCR amplification products using random primers on 22 trees of each species sampled in 11 natural populations. Only two per cent of the amplified fragments exhibited significant frequency differences between the two species and none of them was specific to a species. The nucleotide divergence between the two species estimated with RAPD data was 0.5 per cent in the overall genome and increased to 3.3 per cent in the discriminant regions. Twenty-three informative fragments were cloned and partially sequenced. New primers were derived from these sequences to obtain Sequence Characterized Amplified Region (SCAR) fragments. Southern blot experiments indicated that the SCARs were generally in low copy number in the genome. A search for similarity between SCAR sequences and sequences contained in data banks revealed that three of them corresponded to known DNA sequences.

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