| Publication Type: | Journal Article |
| Year of Publication: | 1999 |
| Authors: | F. Lefort, Douglas G. C. |
| Journal: | Annals of Forest Science |
| Volume: | 56 |
| Pagination: | 259-263 |
| Date Published: | 1999 |
| Abstract: | It is difficult to purify DNA from mature tree leaves at the end of the growing season, because of their thick cell wall, and high content in polysaccharides, phenolic compounds and endonucleases. A simple, fast and efficient method for DNA purification from 100 mg fresh weight leaf samples is described here. It has been developed for extracting DNA from mature leaves of Quercus, Fraxinus Prunus and Acer. The protocol is a modified CTAB (hexadecyltrimethylammonium bromide) method including a combination of |