An efficient micro-method of DNA isolation from mature leaves of four hardwood tree species Acer, Fraxinus, Prunus and Quercus

Publication Type:Journal Article
Year of Publication:1999
Authors:F. Lefort, Douglas G. C.
Journal:Annals of Forest Science
Date Published:1999

It is difficult to purify DNA from mature tree leaves at the end of the growing season, because of their thick cell wall, and high content in polysaccharides, phenolic compounds and endonucleases. A simple, fast and efficient method for DNA purification from 100 mg fresh weight leaf samples is described here. It has been developed for extracting DNA from mature leaves of Quercus, Fraxinus Prunus and Acer. The protocol is a modified CTAB (hexadecyltrimethylammonium bromide) method including a combination of

Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith