Utility of ITS sequence data for phylogenetic reconstruction of Italian Quercus spp

Publication Type:Journal Article
Year of Publication:2005
Authors:R. BELLAROSA, Simeone, M., Papini, A., SCHIRONE, B.
Journal:Molecular Phylogenetics and Evolution
Pagination:355 - 370
Date Published:2005

Nuclear ribosomal DNA sequences encoding the 5.8S RNA and the flanking internal transcribed spacers (ITS1 and ITS2) were used to test the phylogenetic relationships within 12 Italian Quercus taxa (Fagaceae). Hypotheses of sequence orthology are tested by detailed inspection of some basic features of oak ITS sequences (i.e., general patterns of conserved domains, thermodynamic stability and predicted conformation of the secondary structure of transcripts) that also allowed more accurate sequence alignment. Analysis of ITS variation supported three monophyletic groups, corresponding to subg. Cerris, Schlerophyllodrys (=Ilex group sensu Nixon) and Quercus, as proposed by Schwarz [Feddes Rep., Sonderbeih. D, 1-200]. A derivation of the

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