02840nas a2200229   4500008004100000020001400041245016200055210006900217300001200286520205300298653002502351653001302376653002002389653002002409100002302429700002102452700002102473700002002494700002802514700002402542856004402566       2012                        eng d  a1095-831200aA landscape genetics approach reveals ecological-based differentiation in populations of holm oak (<i>Quercus ilex </i>L.) at the northern limit of its range0 alandscape genetics approach reveals ecologicalbased differentiat  an/a-n/a3 aThe holm oak plays a relevant role in the functioning of Mediterranean forests. In the area north of Garda Lake, Italian Prealps, holm oak populations are at the northernmost edge of their distribution. Being peripheral, these populations are of particular interest for ecological, evolutionary and conservation studies. Through an explicit individual-based landscape genetics approach, we addressed the following questions: (1) are levels of genetic variation reduced in these marginal populations compared with central populations?; (2) despite the narrow geographical scale, do individual-based analyses have some power to detect genetic differentiation?; (3) do environmental and/or climatic factors exert a role in shaping patterns of genetic variation and differentiation? Through a Bayesian method, we identified three clusters whose genetic variability can be considered to be of the same order as that recorded in central Quercus ilex populations. Although being geographically very close (< 20 km), the differentiation was statistically significant (P < 0.05) with global F st and ΦPt values of 0.019 and 0.038, respectively. Geography and phylogeography could not be invoked to explain this differentiation. A redundancy discriminant analysis revealed that relevant eco-pedological and climatic features, such as soil depth, aspect, elevation and humidity, were correlated with the observed pattern of differentiation. Toblino was ecologically separated from the other clusters, as it lies on deep soil with subhumid conditions. The differentiation of the Brione–Ranzo–Val Busa cluster appeared to be related to superficial soils and drier conditions, whereas the Nanzone–Padaro cluster was differentiated mainly according to its mid-elevation. Coupling spatial and genetic information on a local scale proved to be effective to investigate the evolutionary and demographic history of peripheral populations. © 2012 The Linnean Society of London, Biological Journal of the Linnean Society, 2012, ••, ••–••.10aBayesian computation10aGeneland10aItalian Prealps10amicrosatellites1 aVernesi, Cristiano1 aRocchini, Duccio1 aPecchioli, Elena1 aNeteler, Markus1 aVendramin, Giovanni, G.1 aPaffetti, Donatella  uhttps://quercus.myspecies.info/node/15101737nas a2200241   4500008004100000020001400041245013600055210006900191260002900260300001200289490000700301520097800308653001901286653002001305653002901325653001701354100001701371700001601388700001501404700001401419700001801433856004401451       2011                        eng d  a1755-099800aTwo highly validated multiplexes (12-plex and 8-plex) for species delimitation and parentage analysis in oaks (<i>Quercus </i>spp.)0 aTwo highly validated multiplexes 12plex and 8plex for species de  bBlackwell Publishing Ltd  a578-5850 v113 aAbstract Multiplex PCR is a fast and cost-effective technique allowing increased genotyping throughput of microsatellites. We developed two multiplexes for Quercus petraea and Q. robur, a 12-plex of EST-SSRs (eSSRs) and an 8-plex of genomic SSRs (gSSRs). We studied the origin of allele calling errors at the human reader and software levels. We showed that the robustness of allele identification can be improved by binning on raw peak sizes prior to genetic data analysis. We checked through simulation the power of these markers for species delimitation and hybrid detection. The resolution achieved with all 20 markers was greatly improved compared to that of previous studies based on a subset of the markers. Preliminary PCR tests suggest that these multiplexes might be useful to study other oak species as well. The strategy used for multiplex microsatellite development (from PCR conditions to the definition of allele calling rules) should be broadly applicable.10amanual binning10amicrosatellites10amolecular identification10aQuercus spp.1 aGuichoux, E.1 aLagache, L.1 aWagner, S.1 aLeger, P.1 aPetit, R., J.  uhttps://quercus.myspecies.info/node/13602094nas a2200205   4500008004100000245007500041210006900116260000900185300001200194490000700206520149700213653001801710653002001728653003001748653002001778653001601798100001401814700001701828856004301845       2002                        eng d00aFactors influencing male mating success in bur oak, Quercus macrocarpa0 aFactors influencing male mating success in bur oak Quercus macro  c2002  a161-1800 v153 aPollen donors of acorns and saplings in a stand of bur oak were identified by paternity exclusion using microsatellite genotype analysis. Here we examine the influence of several factors likely to affect reproductive success of males with wind-dispersed pollen, including distance of pollen donor from maternal tree, genetic relatedness of pollen donor to maternal tree, direction of pollen donor relative to maternal tree, and size of pollen donor (crown volume and trunk diameter). Surprisingly, none of these factors were strongly correlated with fertilization success, although weak but significant correlations with crown volume and distance were found. The slight influence of distance is actually overestimated here, because our analysis necessarily excluded a large proportion of pollinations that were effected by trees outside the stand. The pollen donors outside the stand were not identified, but were more than 150 meters away. Pollination patterns in this stand of bur oak are quite complicated. Seed parents were fertilized by multiple pollen donors within the stand from all directions and as far as 200 m away. These results caution against the use of models of pollination that are based on simple distance or directional parameters because they will not adequately predict gene flow or male reproductive success. Our results further emphasize the importance of genetically identifying successful pollen donors rather than relying on patterns of physical movement of pollen.10afemale choice10aforest genetics10amale reproductive success10amicrosatellites10apollination1 aDow, B.D.1 aAshley, M.V.  uhttps://quercus.myspecies.info/node/4802289nas a2200265   4500008004100000245013400041210006900175300001200244490000700256520147500263653001401738653002801752653001701780653001801797653002001815653001201835100002601847700003001873700001501903700001601918700001301934700002101947700001201968856004301980       2005                        eng d00aGene flow and hybridisation in a mixed oak forest (Quercus pyrenaica Willd. and Quercus petraea (Matts.) Liebl.) in central Spain0 aGene flow and hybridisation in a mixed oak forest Quercus pyrena  a457-4650 v953 aOaks are long-standing models for the study of gene flow and hybridisation. Temperate (Quercus petraea) and sub-Mediterranean (Quercus pyrenaica) oaks coexist in central Spain, showing remarkable differences in population size and structure. Q. petraea has a scattered distribution in central Spain, where it is at one of the southernmost limits of its range, and forms low-density stands; in contrast, Q. pyrenaica is widespread in the region. We selected a mixed population of the two species (approx13 ha, 176 adults and 96 saplings) to compare the patterns of gene flow within each species and the extent of introgression between them. Using five nuclear microsatellite markers, we performed a parentage analysis and found considerable immigration from outside the stand (approx38% for Q. petraea and approx34% for Q. pyrenaica), and estimated average seed-dispersal distances of 42 and 14 m for Q. petraea and Q. pyrenaica, respectively. Introgression between species was also estimated using our microsatellite battery. First, we developed a multivariate discriminant approach and, second, we compared our results with a widely used clustering method (STRUCTURE). Both analyses were consistent with a low level of introgression between Q. petraea and Q. pyrenaica. Indeed, only 15 adult trees, approx8.5%, were identified as putative hybrids when both methods of analysis were combined. Hybrids may be most common in contact zones due merely to physical proximity.10agene flow10agenetic differentiation10ahybrid zones10aintrogression10amicrosatellites10aQuercus1 aValbuena-Carabana, M.1 aGonzalez-Martinez, S., C.1 aSork, V.L.1 aCollada, C.1 aSOTO, A.1 aGoicoechea, P.G.1 aGil, L.  uhttps://quercus.myspecies.info/node/36