02458nas a2200229 4500008004100000020001400041245016200055210006900217300001200286520170600298653001102004653001202015653000902027653001402036653002202050100002302072700002102095700002002116700002302136700002502159856004402184 2013 eng d a1095-833900aApplication of plastid and nuclear markers to DNA barcoding of Euro-Mediterranean oaks (Quercus, Fagaceae): problems, prospects and phylogenetic implications0 aApplication of plastid and nuclear markers to DNA barcoding of E an/a-n/a3 aDNA barcoding, a species identification system based on sequences from a short, standardized DNA region, has emerged recently as a new tool for taxonomists. We investigated the discriminatory power of a subset of highly variable proposed plant barcoding loci (matK, trnH-psbA, ITS2) in Quercus, a taxonomically complex tree genus of global importance. The research included all currently recognized species and some major variants of the Mediterranean region and Europe (32 taxa) and 17 East Asian and North American species used for comparison. Based on sequence character state, we assigned unique plastid haplotypes to 40.8% of the investigated species; ITS2 increased the resolution up to 87.8% of total taxa. Nevertheless, unsuccessful genetic distance-based discrimination questioned the potential efficiency of correct species identification for future studies. Most species appeared to be nonmonophyletic in parallel phylogenetic tests. Three subgeneric groups were outlined, with different rates of within-group variability and geographical differentiation. Members of one of these groups (corresponding to the Eurasian Group Ilex) were paraphyletic to Group Quercus from the New and Old World and the Eurasian Group Cerris. The data gathered indicate that barcoding markers may help to identify closely related species clusters and contribute to the inference of major diversification and evolutionary patterns in oaks, but the methodology per se appears to be of limited efficacy in defining species limits, unless we make a profound revision of traditional Quercus taxonomic categories. © 2013 The Linnean Society of London, Botanical Journal of the Linnean Society, 2013, 00, 000–000.10acerris10aecology10ailex10aphylogeny10aregional sampling1 aSimeone, Marco, C.1 aPiredda, Roberta1 aPapini, Alessio1 aVessella, Federico1 aSchirone, Bartolomeo uhttps://quercus.myspecies.info/node/15701166nas a2200205 4500008004100000245012500041210006900166300001200235490000700247520054500254653001100799653000900810653002300819653001200842653001100854100001800865700001500883700001800898856004400916 2011 eng d00aCytogenetic studies on Quercus L. (Fagaceae) species belonging to Ilex and Cerris section in Turkey0 aCytogenetic studies on iQuercus iL Fagaceae species belonging to a297-3010 v643 aA detailed karyotype analyses of the five species of Quercus L. (Q. coccifera L., Q. ilex L., Q. aucheriJaub. & Spach, Q. cerris L. and Q. ithaburensis Decne subsp. macrolepsis (Kotschy) Hedge & Yalt.) belonging toIlex and Cerris section were examined. The somatic chromosome number of all studied taxa 2n = 24 was found.The karyotypes of investigated species showed the great similarity with the chromosome length and the positionof centromere. Chromosome number and detailed karyotype of these species were first time counted from Turkey.10acerris10ailex10aKaryotype analyses10aQuercus10aTurkey1 aAykut, Yilmaz1 aUslu, Emel1 aBabaç, Tekin uhttps://quercus.myspecies.info/node/134