@article {174, title = {Single-nucleotide polymorphism discovery and validation in high density SNP array for genetic analysis in European white oaks}, journal = {Molecular Ecology Resources}, year = {2015}, pages = {n/a-n/a}, abstract = {

An Illumina Infinium SNP genotyping array was constructed for European white oaks. Six individuals of Quercus petraea and Q. robur were considered for SNP discovery using both previously obtained Sanger sequences across 676 gene regions (1371 in vitro SNPs) and Roche 454 technology sequences from 5,112 contigs (6,542 putative in silico SNPs). The 7,913 SNPs were genotyped across the six parental individuals, full-sib progenies (one within each species and two interspecific crosses between Q. petraea and Q. robur), and three natural populations from south-western France that included two additional interfertile white oak species (Q. pubescens and Q. pyrenaica). The genotyping success rate in mapping populations was 80.4\% overall and 72.4\% for polymorphic SNPs. In natural populations, these figures were lower (54.8\% and 51.9\%, respectively). Illumina genotype clusters with compression (shift of clusters on the normalised X-axis) were detected in \ 25\% of the successfully genotyped SNPs, and may be due to the presence of paralogues. Compressed clusters were significantly more frequent for SNPs showing a priori incorrect Illumina genotypes, suggesting that they should be considered with caution or discarded. Altogether, these results show a high experimental error rate for the Infinium array (between 15\% to 20\% of SNPs potentially unreliable, and 10\% when excluding all compressed clusters), and recommendations are proposed when applying this type of high-throughput technique. Finally, results on diversity levels and shared polymorphisms across targeted white oaks and more distant species of the Quercus genus are discussed, and perspectives for future comparative studies are proposed. This article is protected by copyright. All rights reserved.

}, author = {Lepoittevin, C. and Bodenes, C. and Chancerel, E. and Villate, L. and Lang, T. and Lesur, I. and Boury, C. and Ehrenmann, F. and Zelenica, D. and Boland, A. and Besse, C. and Garnier-G{\'E}R{\'E}, P. and Plomion, C. and Kremer, A.} } @article {132, title = {Strength, diversity and plasticity of postmating reproductive barriers between two hybridizing oak species (Quercus robur L. and Quercus petraea (Matt) Liebl.)}, journal = {Journal of Evolutionary BiologyJournal of Evolutionary Biology}, year = {2011}, pages = {no-no}, publisher = {Blackwell Publishing Ltd}, abstract = {Very little is known about the nature and strength of reproductive isolation (RI) in Quercus species, despite extensive research on the estimation and evolutionary significance of hybridization rates. We characterized postmating pre- and postzygotic RI between two hybridizing oak species, Quercus robur and Quercus petraea, using a large set of controlled crosses between different genotypes. Various traits potentially associated with reproductive barriers were quantified at several life history stages, from pollen{\textendash}pistil interactions to seed set and progeny fitness-related traits. Results indicate strong intrinsic postmating prezygotic barriers, with significant barriers also at the postzygotic level, but relatively weaker extrinsic barriers on early hybrid fitness measures assessed in controlled conditions. Using general linear modelling of common garden data with clonal replicates, we showed that most traits exhibited important genotypic differences, as well as different levels of sensitivity to micro-environmental heterogeneity. These new findings suggest a large potential genetic diversity and plasticity of reproductive barriers and are confronted with hybridization evidence in these oak species.}, keywords = {controlled crosses, ecological speciation, pollen{\textendash}pistil interactions, prezygotic barriers, Quercus petraea, Quercus robur, reproductive isolation}, isbn = {1420-9101}, author = {Abadie, P. and Roussel, G. and Dencausse, B. and Bonnet, C. and Bertocchi, E. and Louvet, J. M. and Kremer, A. and Garnier-G{\'E}R{\'E}, P.} }