TY - JOUR T1 - Utility of ITS sequence data for phylogenetic reconstruction of Italian Quercus spp JF - Molecular Phylogenetics and Evolution Y1 - 2005 A1 - BELLAROSA, R. A1 - Simeone, M. A1 - Papini, A. A1 - SCHIRONE, B. SP - 355 EP - 370 AB - Nuclear ribosomal DNA sequences encoding the 5.8S RNA and the flanking internal transcribed spacers (ITS1 and ITS2) were used to test the phylogenetic relationships within 12 Italian Quercus taxa (Fagaceae). Hypotheses of sequence orthology are tested by detailed inspection of some basic features of oak ITS sequences (i.e., general patterns of conserved domains, thermodynamic stability and predicted conformation of the secondary structure of transcripts) that also allowed more accurate sequence alignment. Analysis of ITS variation supported three monophyletic groups, corresponding to subg. Cerris, Schlerophyllodrys (=Ilex group sensu Nixon) and Quercus, as proposed by Schwarz [Feddes Rep., Sonderbeih. D, 1-200]. A derivation of the VL - 34 N1 - doi:10.1016/j.ympev.2004.10.014 ER -